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Monoclonal antibodies against beta-galactoside-binding lectin of chick embryo recognizes conformational change of the antigen
Authors:Y Oda  K Kasai
Affiliation:Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Kanagawa 199-01, Japan
Abstract:Monoclonal antibodies against chick embryonic beta-galactoside-binding lectin were obtained. One of the monoclonal antibodies was ineffective in Western blotting and seemed to be unable to bind the SDS-denatured lectin. When the native lectin was dotted on a nitrocellulose filter and subjected to denaturation by treatment with SDS, urea or heat, binding of this antibody no longer occurred, though other monoclonal antibodies bound normally. This antibody seems to have been raised against an epitope which is destroyed upon denaturation.
Keywords:PBS  0.01 M sodium phosphate buffer pH 7.2 containing 0.15 M NaCl  PBS-Tween  PBS containing 0.3 % Tween 20  HRP  horseradish peroxidase  SDS  sodium dodecyl sulfate
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