Characterization of a new repetitive sequence that is enriched on microchromosomes of turkey |
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| Authors: | A J M Matzke F Varga P Gruendler I Unfried H Berger B Mayr M A Matzke |
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| Institution: | (1) Institute for Molecular Biology, Austrian Academy of Sciences, Billrothstrasse 11, A-5020 Salzburg, Austria;(2) Ludwig Boltzmann Research Unit for Clinical and Experimental Osteology, Hanusch Hospital, Heinrich-Collinstrasse 30, A-1140 Vienna, Austria;(3) Department of Cytology and Genetics, Institute for Botany, Rennweg 14, A-1030 Vienna, Austria;(4) Institute for Animal Breeding and Genetics, Linke Bahngasse 11, A-1030 Vienna, Austria |
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| Abstract: | We cloned and characterized a new highly repetitive, species-specific DNA sequence from turkey (Meleagris gallopavo). This repeat family, which accounts for approximately 5% of the turkey genome, consists of a 41 bp repeated element that is present in tandem arrays longer than 23 kb. In situ hybridization to turkey metaphase chromosomes (2n=80) demonstrated that this sequence was located primarily on certain microchromosomes: approximately one-third of the 66 microchromosomes showed a positive signal. With respect to the macrochromosomes, hybridization was seen only in a pericentric position on nos. 2 and 3. The turkey microchromosome (TM) sequence shares motifs (alternating A3–5 and T3–5 clusters separated by 6–8 bp) that have been found previously in other avian tandemly repeated elements, e.g. a chicken microchromosome sequence, and W (female) chromosome-specific sequences of chicken and turkey. However, the TM sequence does not cross-hybridize under moderately stringent conditions with these other sequence. The spread and amplification of related repetitive sequence elements on microchromosomes and W chromosomes is discussed.by E.R. Schmidt |
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