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Sequence and Structure Analysis of Cryptic Plasmid pN30 from Oil-Oxidizing Strain <Emphasis Type="Italic">Rhodococcus erythropolis</Emphasis> 30
Authors:L E Ryabchenko  A D Novikov  P N Golyshin  A S Yanenko
Institution:(1) State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, 117545, Russia;(2) German Research Centre for Biotechnology, Braunschweig, D-38124, Germany
Abstract:Nucleotide sequence of cryptic plasmid pN30 from a Rhodococcus erythropolis 30 soil isolate was determined. Plasmid DNA consists of 5403 nucleotide pairs and contains about 62% GC pairs, which is typical of Rhodococcus DNA. No significant homology was determined between the pN30 DNA sequence and those of known plasmids. Computer-aided analysis of pN30 sequence revealed open reading frames that encode proteins strongly homologous to replicative proteins encoded by small cryptic plasmids of different actinomycetes.
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