New insights into the diversity and evolution of the archaeal mobilome from three complete genomes of Saccharolobus shibatae |
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Authors: | Sofia Medvedeva David Brandt Virginija Cvirkaite-Krupovic Ying Liu Konstantin Severinov Sonoko Ishino Yoshizumi Ishino David Prangishvili Jörn Kalinowski Mart Krupovic |
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Institution: | 1. Archaeal Virology Unit, Institut Pasteur, Paris, 75015 France
Center of Life Science, Skolkovo Institute of Science and Technology, Moscow, 121205 Russia;2. Center for Biotechnology, Universität Bielefeld, Bielefeld, 33615 Germany;3. Archaeal Virology Unit, Institut Pasteur, Paris, 75015 France;4. Center of Life Science, Skolkovo Institute of Science and Technology, Moscow, 121205 Russia
Waksman Institute, Rutgers University, Piscataway, NJ, 08854 USA
Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, 123182 Russia;5. Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395 Japan |
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Abstract: | Saccharolobus (formerly Sulfolobus) shibatae B12, isolated from a hot spring in Beppu, Japan in 1982, is one of the first hyperthermophilic and acidophilic archaeal species to be discovered. It serves as a natural host to the extensively studied spindle-shaped virus SSV1, a prototype of the Fuselloviridae family. Two additional Sa. shibatae strains, BEU9 and S38A, sensitive to viruses of the families Lipothrixviridae and Portogloboviridae, respectively, have been isolated more recently. However, none of the strains has been fully sequenced, limiting their utility for studies on archaeal biology and virus–host interactions. Here, we present the complete genome sequences of all three Sa. shibatae strains and explore the rich diversity of their integrated mobile genetic elements (MGE), including transposable insertion sequences, integrative and conjugative elements, plasmids, and viruses, some of which were also detected in the extrachromosomal form. Analysis of related MGEs in other Sulfolobales species and patterns of CRISPR spacer targeting revealed a complex network of MGE distributions, involving horizontal spread and relatively frequent host switching by MGEs over large phylogenetic distances, involving species of the genera Saccharolobus, Sulfurisphaera and Acidianus. Furthermore, we characterize a remarkable case of a virus-to-plasmid transition, whereby a fusellovirus has lost the genes encoding for the capsid proteins, while retaining the replication module, effectively becoming a plasmid. |
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