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Azole-resistant Aspergillus fumigatus is highly prevalent in the environment of Vietnam,with marked variability by land use type |
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| Authors: | Tra-My N. Duong Thanh-Van Le Khanh-Linh H. Tran Phuong-Tuyen Nguyen Bich-Phuong T. Nguyen Thu-Anh Nguyen Huong-Lan P. Nguyen Bich-Ngoc T. Nguyen Matthew C. Fisher Johanna Rhodes Guy Marks Greg J. Fox Sharon C.-A. Chen Michael G. Walsh Vanessa R. Barrs Jessica Talbot Catriona L. Halliday Tania C. Sorrell Jeremy N. Day Justin Beardsley |
| Affiliation: | 1. Faculty of Medicine and Health, The University of Sydney, Sydney, 2145 Australia Oxford University Clinical Research Unit, Ho Chi Minh City, 70000 Vietnam;2. Oxford University Clinical Research Unit, Ho Chi Minh City, 70000 Vietnam;3. Woolcock Institute of Medical Research, Hanoi, 10000 Vietnam;4. Hospital for Tropical Diseases, Ho Chi Minh, 70000 Vietnam;5. National Lung Hospital, Hanoi, 10000 Vietnam Hanoi Medical University, Hanoi, 10000 Vietnam;6. MRC Centre for Global Infectious Disease Analysis, Imperial College London, London, W2 1NY UK;7. Faculty of Medicine and Health, The University of Sydney, Sydney, 2145 Australia Woolcock Institute of Medical Research, Hanoi, 10000 Vietnam;8. Marie Bashir Institute for Infectious Diseases and Biosecurity, The University of Sydney, Sydney, 2145 Australia Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, NSW Health Pathology, Westmead Hospital, Sydney, 2145 Australia;9. Faculty of Medicine and Health, The University of Sydney, Sydney, 2145 Australia Marie Bashir Institute for Infectious Diseases and Biosecurity, The University of Sydney, Sydney, 2145 Australia;10. Marie Bashir Institute for Infectious Diseases and Biosecurity, The University of Sydney, Sydney, 2145 Australia Department of Veterinary Clinical Sciences, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon Tong, Hong Kong;11. Faculty of Veterinary Science, The University of Sydney, Sydney, 2145 Australia;12. Faculty of Medicine and Health, The University of Sydney, Sydney, 2145 Australia Marie Bashir Institute for Infectious Diseases and Biosecurity, The University of Sydney, Sydney, 2145 Australia Westmead Institute for Medical Research, Westmead, Sydney, 2145 Australia;13. Oxford University Clinical Research Unit, Ho Chi Minh City, 70000 Vietnam Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, OX3 7FZ UK;14. Faculty of Medicine and Health, The University of Sydney, Sydney, 2145 Australia |
| Abstract: | Azole-resistant environmental Aspergillus fumigatus presents a threat to public health but the extent of this threat in Southeast Asia is poorly described. We conducted environmental surveillance in the Mekong Delta region of Vietnam, collecting air and ground samples across key land-use types, and determined antifungal susceptibilities of Aspergillus section Fumigati (ASF) isolates and azole concentrations in soils. Of 119 ASF isolates, 55% were resistant (or non-wild type) to itraconazole, 65% to posaconazole and 50% to voriconazole. Azole resistance was more frequent in A. fumigatus sensu stricto isolates (95%) than other ASF species (32%). Resistant isolates and agricultural azole residues were overrepresented in samples from cultivated land. cyp51A gene sequence analysis showed 38/56 resistant A. fumigatus sensu stricto isolates carried known resistance mutations, with TR34/L98H most frequent (34/38). |
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