Ultrastructure of in vitro formed actin-anti-actin immune complexes |
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| Authors: | G Utter P Biberfeld R Norberg R Thorstensson A Fagraeus |
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| Affiliation: | Department of Immunology, National Bacteriological Laboratory, S-105 21 Stockholm, Sweden |
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| Abstract: | The reactions of five human smooth-muscle-antibody positive sera with F-actin from rabbit skeletal muscle were studied by electronmicroscopy with the negative contrasting technique. The immune complexes consisted of parallel arrays of actin filaments cross-linked by antibodies. Small complexes had a ladder-like appearance on some regions suggesting a periodic antibody binding. The antibodies were observed directly and by the aid of a ferritin-conjugated anti-human Ig. |
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