Inhibition of galectin-3 mediated cellular interactions by pectic polysaccharides from dietary sources |
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Authors: | U V Sathisha Smitha Jayaram M A Harish Nayaka Shylaja M Dharmesh |
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Institution: | (1) Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore, 570 020, India |
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Abstract: | Pectic polysaccharides from dietary sources such as Decalepis hamiltonii—swallow root (SRPP), Hemidesmus indicus (HPP), Nigella sativa—black cumin (BCPP), Andrographis serpyllifolia—(APP), Zingiber officinale—ginger (GRPP) and, citrus pectin (CPP) were examined for galectin inhibitory activity. Inhibition of (a) galectin-3 of MDA-MB-231
cells induced hemagglutination of red blood cells; (b) galectin-3 mediated interaction between normal/metastatic human buccal
cells (NBC)/(MBC) and; (c) invasion of MDA-MB-231 and MBC in the invasive chamber was assessed. Results indicated that SRPP
inhibited hemagglutination at Minimum Inhibitory Concentration (MIC) of 1.86 μg ml−1 equivalent of carbohydrate as apposed to those of BCPP (130 μg ml−1), APP (40 μg ml−1), HPP (40 μg ml−1) and CPP (25 μg ml−1). GRPP even at concentration >1–6 mg ml−1 did not inhibit agglutination. Also SRPP showed ∼15 and 2 fold potent anti hemagglutination activity relative to that of
galectin-3 specific sugars—galactose (MIC-27.1 μg ml−1) and lactose (MIC-4.16 μg ml−1) respectively. Further, SRPP at 10 μg ml−1 inhibited agglutination of NBC by galectin-3 of MDA-MB-231 cells. Modified swallow root pectic polysaccharide (MSRPP) of
50 kDa retained anti hemagglutination activity (MIC of 1.03 μg ml−1) and inhibited MDA-MB-231 and MBC invasion by 73 and 50% with an IC50 of 136 and 200 μg ml−1 respectively. Both SRPP and MSRPP induced apoptosis up to 80% at 100 μg ml−1 concentration by activating ∼2 and 8 folds of Caspase-3 activity. Sugar composition analysis and its correlation with the
galectin inhibitory property indicated that pectic polysaccharides with higher arabinose and galactose content—arabinogalactan
inhibited hemagglutination significantly. |
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Keywords: | Galectin-3 Pectic polysaccharide Hemagglutination Anti metastatic activity Arabinogalactan Modified pectic polysaccharide |
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