Interrelationship between pH-dependent uptake of bromophenol blue by intact Saccharomyces cerevisiae cells and their viability after dehydration

Summary An inverse linear relationship (n=16; r=0.929; 1-P=0.001) was established between the viability of dehydrated-rehydrated yeasts and the uptake of a weak acid, bromophenol blue, by samples of intact cells. The dependence indicates the fact that yeasts that are more resistant to dehydration have lower intracellular pH values, and hence can be used to predict the viability of yeasts to be dried.