Enhanced hydrogen production from aromatic acids by immobilized cells of Rhodopseudomonas palustris |
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Authors: | J. Fiβler G. W. Kohring F. Giffhorn |
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Affiliation: | (1) Angewandte Mikrobiologie, University:at des Saarlandes, FB 13.3, Im Stadtwald Geb. 2, Postfach 151150, D-66041 Saarbrücken, Germany |
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Abstract: | Cells of the purple non-sulphur bacterium Rhodopseudomonas palustris DSM 131 were immobilized in agar, agarose, -carrageenan or sodium alginate gel. With alginate beads, prepared by an emulsion technique, and an optimal cell load of 10 mg dry weight/ml gel, the hydrogen production from aromatic acids was doubled as compared to that resulting from liquid cultures. Hydrogen yields of 60%, 57%, 86% or 88% of the maximal theoretical value were obtained from mandelate, benzoylformate, cinnamate or benzoate respectively. Benzoate concentrations above 16.5 mM were inhibitory. During a period of 55 days the process of hydrogen evolution with immobilized cells was repeated in five cycles with slowly decreasing efficiency. |
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