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Molecular karyotype analysis of Perkinsus atlanticus (Phylum Perkinsozoa) by pulsed field gel electrophoresis |
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| Authors: | Leonor Teles-Grilo M Duarte Sérgio M Tato-Costa Joana Gaspar-Maia Alexandre Oliveira Carla Rocha António A Marques Américo Cordeiro-da-Silva Anabela Azevedo Carlos |
| Affiliation: | aMolecular Genetics, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Lg. Abel Salazar no. 2, P-4099-003 Porto, Portugal bCell Biology Laboratories, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Lg. Abel Salazar no. 2, P-4099-003 Porto, Portugal cCIIMAR, University of Porto, P-4050-123 Porto, Portugal dBiochemistry Laboratory, Faculty of Pharmacy, University of Porto, P-4050-047 Porto, Portugal eInstitute of Molecular and Cellular Biology (IBMC), University of Porto, P-4150-180 Porto, Portugal |
| Abstract: | Perkinsus atlanticus is a pathogenic protist that infects the clam Ruditapes decussatus. Although it was recently proposed that the genus Perkinsus belongs to a new phylum, Perkinsozoa, in the infra-kingdom Alveolata, there remain different opinions about whether this genus should form a phylum on its own and consequently divergent views about its taxonomic characterization. In this work, we have identified nine chromosomes by pulsed field gel electrophoresis (PFGE) combined with densitometry analysis. The obtained karyotype of Perkinsus atlanticus, like that of other early branches of the dinoflagellate lineage, displays a more conventional chromosome organization, different from that of most dinoflagellates. |
| Keywords: | Perkinsus atlanticus Perkinsozoa Alveolata Molecular karyotype |
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