Effect of abscisic acid on photosynthetic parameters during <Emphasis Type="Italic">ex vitro</Emphasis> transfer of micropropagated tobacco plantlets |
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Authors: | J Pospíšilová H Synková D Haisel P Baťková |
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Institution: | (1) Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Na Karlovce 1, CZ-16000 Prague, Czech Republic;(2) Faculty of Science, Charles University in Prague, Vinièná 5, CZ-12844 Prague, Czech Republic |
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Abstract: | The aim of this research was to determine whether exogenous abscisic acid (ABA) applied immediately after ex vitro transfer of in vitro grown plants can improve their acclimatization. Tobacco (Nicotiana tabacum L.) plantlets were transferred into pots with Perlite initially moistened either by water or 50 μM ABA solution and they
were grown under low (LI) or high (HI) irradiance of 150 and 700 μmol m−2 s−1, respectively. Endogenous content of ABA in tobacco leaves increased considerably after ABA application and even more in
plants grown under HI. Stomatal conductance, transpiration rate and net photosynthetic rate decreased considerably 1 d after
ex vitro transfer and increased thereafter. The gas exchange parameters were further decreased by ABA application and so wilting of
these plants was limited. Chlorophyll (a+b) and β-carotene contents were higher in ABA-treated plants, but the content of xanthophyll cycle pigments was not increased.
However, the degree of xanthophyll cycle pigments deepoxidation was decreased what also suggested less stress in ABA-treated
plants. No dramatic changes in most chlorophyll a fluorescence parameters after ex vitro transfer suggested that the plants did not suffer from restriction of electron transport or photosystem damage. |
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Keywords: | Additional" target="_blank">Additional carotenoids chlorophyll contents chlorophyll fluorescence net photosynthetic rate Nicotiana tabacum stomatal conductance transpiration rate xanthophyll cycle pigments |
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