Vinculin and Rab5 Complex Is Requited for Uptake of Staphyrococcus aureus and Interleukin-6 Expression |
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| Authors: | Makoto Hagiwara Eitoyo Kokubu Shinsuke Sugiura Toshinori Komatsu Hiroyuki Tada Ryutaro Isoda Naomi Tanigawa Yoshiko Kato Naoyuki Ishida Kaoru Kobayashi Misako Nakashima Kazuyuki Ishihara Kenji Matsushita |
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| Affiliation: | 1. Department of Oral Disease Research, National Center for Geriatrics and Gerontology, Obu, Aichi, Japan.; 2. Department of Microbiology, Tokyo Dental College, Chiba, Japan.; University of California Merced, United States of America, |
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| Abstract: | Vinculin, a 116-kDa membrane cytoskeletal protein, is an important molecule for cell adhesion; however, little is known about its other cellular functions. Here, we demonstrated that vinculin binds to Rab5 and is required for Staphylococcus aureus (S. aureus) uptake in cells. Viunculin directly bound to Rab5 and enhanced the activation of S. aureus uptake. Over-expression of active vinculin mutants enhanced S. aureus uptake, whereas over-expression of an inactive vinculin mutant decreased S. aureus uptake. Vinculin bound to Rab5 at the N-terminal region (1-258) of vinculin. Vinculin and Rab5 were involved in the S. aureus-induced phosphorylation of MAP kinases (p38, Erk, and JNK) and IL-6 expression. Finally, vinculin and Rab5 knockdown reduced infection of S. aureus, phosphorylation of MAPKs and IL-6 expression in murine lungs. Our results suggest that vinculin binds to Rab5 and that these two molecules cooperatively enhance bacterial infection and the inflammatory response. |
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