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Genome Sequence and Analysis of Buzura suppressaria Nucleopolyhedrovirus: A Group II Alphabaculovirus
Authors:Zheng Zhu  Feifei Yin  Xiaoping Liu  Dianhai Hou  Jun Wang  Lei Zhang  Basil Arif  Hualin Wang  Fei Deng  Zhihong Hu
Affiliation:1. State Key Laboratory of Virology and China Center for Virus Culture Collection, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.; 2. Canadian Forest Service, Great Lakes Forestry Centre, Sault Ste Marie, Ontario, Canada.; Wuhan Bioengineering Institute, China,
Abstract:The genome of Buzura suppressaria nucleopolyhedrovirus (BusuNPV) was sequenced by 454 pyrosequencing technology. The size of the genome is 120,420 bp with 36.8% G+C content. It contains 127 hypothetical open reading frames (ORFs) covering 90.7% of the genome and includes the 37 conserved baculovirus core genes, 84 genes found in other baculoviruses, and 6 unique ORFs. No typical baculoviral homologous repeats (hrs) were present but the genome contained a region of repeated sequences. Gene Parity Plots revealed a 28.8 kb region conserved among the alpha- and beta-baculoviruses. Overall comparisons of BusuNPV to other baculoviruses point to a distinct species in group II Alphabaculovirus.
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