Use of manure concentrations of ash or specific minerals and nitrogen to estimate loss of volatile nitrogen from manure incubated under laboratory conditions

Changes in the ratio of volatile N to non-volatile specific minerals or total ash in manure can potentially be used to estimate losses of N from manure. The aim of this study was to evaluate the accuracy of using specific minerals (P, K, Ca, Mg, and Na) or total ash as markers to estimate volatile N losses from incubated manure slurries. Holstein cows were fed diets with the forage as predominantly corn silage (CS) or alfalfa silage (AS) or a diet identical to the CS diet except 5 g/kg urea was added (CSU). Total output of urine and feces were measured and used to prepare two types of slurry mixtures (all slurry mixtures contained 1200 g of manure per tray): (1) as excreted (AsEx) and (2) AsEx + sand (SAND). The SAND slurry was designed to mimic manure from barns that used sand as a bedding material. Feces and urine were mixed in the same proportion as excreted by the cows for both slurry types and 240 g of sand were added to the SAND slurries. Initial and final (3 d incubation period) slurry weights and concentrations of N, ash, and specific minerals were measured to calculate loss of volatile N. Losses of volatile N from the slurries were estimated as: (N intake ? N milk) ? N/ash × (ash intake ? ash milk)], where N and ash intake = g/d consumed by the cow; N and ash milk = g/d secreted in milk; and N/ash = ratio of N and ash concentrations in slurry samples at 3 d. To evaluate specific minerals as markers, the same equation was used except that Ca, K, Mg, Na or P replaced the ash term. Measured N losses from the AsEx slurries were 0.44, 0.62, and 1.03 g/3 d for the AS, CS, and CSU treatments and estimated N losses (using N/ash) were 0.29, 0.49, and 1.14 g/3 d. Estimated losses were less than measured losses for cows with negative N balance and greater than measured losses for cows in positive N balance. For AsEx slurries, the use of N to specific mineral ratios was generally less accurate than using the N/ash ratio. Estimated N losses from SAND slurries were extremely inaccurate for all markers. The use of the N/ash ratio to estimate N volatilization from manure shows promise but markers that are in appreciable concentrations in bedding material will not be accurate.