Detection of β-galactofuranosidase production by Penicillium and Aspergillus species using 4-nitrophenyl β-D-galactofuranoside |
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| Authors: | M.A. COUSIN S. NOTERMANS P. HOOGERHOUT J.H. VAN BOOM |
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| Affiliation: | Purdue University, Department of Food Science, West Lafayette, Indiana 47907, USA;National Institute of Public Health and Environmental Protection, P.O. Box 1, 3720 BA, Bilthoven, The Netherlands;DGGorlaeus Laboratories, University of Leiden, P.O. Box 9502, 2300 RA, Leiden, The Netherlands |
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| Abstract: | An assay was developed for detecting β-galactofuranosidase produced by Penicillium and Aspergillus spp. The substrate for the assay, 4-nitrophenyl β-D-galactofura-noside, was synthesized from penta-O-acetyl-β-D-galactofuranose and 4-nitrophenol by a tin chloride catalyzed reaction followed by O-deacetylation. Aspergillus spp. produced only small quantities of β-galactofuranosidase during 30 d at 25°C. Only the biverticillate Penicillium spp. ( P. funiculosum, P. islandicum, P. rubrum and P. tardum ) produced substantial β-galactofuranosidase after 1–4 weeks at 25°C. No extracellular antigens of these four Penicillium spp. could be detected in culture filtrates by the sandwich ELISA technique when antibodies to the extracellular β-galactofuranoside-containing polysaccharide antigen of P. digitatum was used. Antigens to all other Penicillium and Aspergillus spp. were easily detected in their culture filtrates. |
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