Untersuchungen über das Pollenschlauchwachstum von Pisum sativum und einiger mutanten |
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Authors: | Gisela Wolff |
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Institution: | (1) Institut für Genetik der Universität Bonn, BRD |
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Abstract: | Summary When the pollen tubes of Pisum sativum (initial line) and of its mutants are grown on a standard medium containing only sucrose, boric acid and agar-agar, no difference in maximum length was observed. But, while pollen tubes of the initial line took nine hours to reach this length, pollen tubes of the mutants needed only six hours. Growth seems to be faster in pollen tubes of the mutants than in those of the initial line.Further investigations examined the influence of twenty-one amino acids on pollen tube growth. With the initial line, these substances can be classified into three groups: those that promote pollen tube growth; those which have no influence upon its growth; and those which reduce its growth. The amino acids of each group are characterized by special structural properties. Those amino acids which accelerate pollen tube growth of the initial line show variable effects on the pollen tubes of the mutants. In some cases the same behaviour of pollen tubes can be observed whether amino acids are added or not, in others the addition of amino acids has a positive effect on pollen tube growth, though less than on pollen tubes of the initial line, and in a single case the addition of an amino acid is followed by a negative effect on growth. |
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