Molecular diversity of <Emphasis Type="Italic">Frankia</Emphasis> from root nodules of <Emphasis Type="Italic">Hippophae salicifolia</Emphasis> D.Don found in Sikkim |
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| Authors: | Anindita Khan David D Myrold Arvind Kumar Misra |
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| Institution: | (1) Molecular Genetics Laboratory, Department of Botany, North Eastern Hill University, Shillong, 793 022, India;(2) Department of Crop and Soil Science, Oregon State University, Corvallis, OR 97331, USA |
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| Abstract: | Molecular diversity of Frankia was assessed directly from the root nodules of Hippophae salicifolia naturally occurring in North Sikkim. Amplicon restriction patterns (ARPs) were developed by digesting 16S-ITS-23S amplicons
with RsaI. Three ARPs were detected, showing diversity among strains of Frankia that nodulate Hippophae. This was confirmed by sequencing one amplicon each for the three ARPs. Therefore, ARP can be used as a tool for screening
amplicons for nucleotide sequencing. |
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| Keywords: | Amplicon restriction pattern Frankia Hippophae ITS |
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