A simple and sensitive apparatus for continuous monitoring of orthophosphate in the presence of acid-labile compounds

A new technique is described for obtaining reproducible transient-state isoelectric focusing patterns of human pancreatic secretory proteins. This method is excellent for preservation of the proteins for further analysis because of the avoidance of urea, the short running times, and low temperatures. The protein patterns were analyzed on a point-by-point basis by a computer using an extension of our previously published method (Allan, B. J., Kirk, J., and White, T. T., 1978, Biochem. Biophys. Res. Commun., 85, 1239–1246). It was possible with these techniques to subtract various types of backgrounds and to construct average densitometric tracings for statistical analysis.