| 猪C1q的分离纯化与鉴定 |
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| 引用本文： | 任建卫,杨彦.猪C1q的分离纯化与鉴定[J].中国生物化学与分子生物学报,1996,12(1):68-72. |
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| 作者姓名： | 任建卫 杨彦 |
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| 作者单位： | 南开大学分子生物学研究所 |
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| 摘 要： | 猪Ｃ１ｑ由猪血清通过ＰＥＧ沉淀优球蛋白、低离子强度透析沉淀、ＩｇＧ－Ｓｅｐｈａｒｏｓｅ４８亲和层析、ＳｅｐｈａｄｅｘＧ－２００凝胶层析等步骤分离纯化，每３００ｍｌ血清可制得９．８ｍｇＣ１ｑ，产率为４６．７％。纯化的猪Ｃ１ｑ在ＳＤＳ－ＰＡＧＥ上显示出三条染色带，分别在２９、２６、２２ｋＤ处，薄层扫描结果表明纯度达９１％；纯化的Ｃ１ｑ保持了较高活性，终浓度为４μｇ／ｍｌ时仍可使致敏的绵羊红细胞出现明显的凝集现象。猪Ｃ１ｑ－ＥＬＩＳＡ结果表明，动物Ｃ１ｑ代替人Ｃ１ｑ应用于临床检测是可行的。
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| 关 键 词： | 猪C1q IgG－Sepharose4B亲和层析 |
| 收稿时间： | 1996-02-20 |
Purification and Characterization of Porcine Clq |
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| Ren Jian-Wei,Yang Yan.Purification and Characterization of Porcine Clq[J].Chinese Journal of Biochemistry and Molecular Biology,1996,12(1):68-72. |
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| Authors: | Ren Jian-Wei Yang Yan |
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| Institution: | (Institute for Molecular Biology,Nankai University,Tianjin 300071 |
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| Abstract: | Porcine C1q was purified from porcine serum by means of euglobulin precipitation by PEG, low ionic strength precipitation, IgG-Sepharose 4B affinity chromatography and Sephadex G-200 chromatography. 9. 8 mg C1q could be obtained from 300 ml serum,the yield was 46. 7% and the purity was as high as 91%. The results of sheep erythrocytes agglutination test and C1qELISA showed that the purified Clq kept a high activity. |
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| Keywords: | Porcine C1q IgG-Sepharose 4B affinity chromatography |
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