Transfection of human HSP27 in rodent cells: Absence of compensatory regulation between small heat shock proteins |
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Authors: | Robert V Blackburn Sandra S Galoforo Christine M Berns Peter M Corry and Yong J Lee |
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Institution: | Department of Radiation Oncology, William Beaumont Hospital 3601 West Thirteen Mile Road, Royal Oak, Michigan, U.S.A. |
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Abstract: | - 1. 1. We examined rodent cells transfected with an expression plasmid encoding a human small heat shock protein for possible compensatory expression of endogenous heat shock genes. For these investigations, human hsp27 was transfected into CHO cells which express endogenous HSP25.
- 2. 2. Both endogenous HSP25 and transfected HSP27 were expressed and multiple phosphorylated isoforms were detected upon exposure to thermal stress.
- 3. 3. Levels of endogenous HSP70 and HSP25 did not appear to be altered by expression of the heterologous heat shock protein.
- 4. 4. These results suggest that compensatory interactions are not exhibited in the expression of the heat shock genes examined, and that independent regulation may exist not only between the large and small heat shock proteins, but also between individual small heat shock proteins as well.
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Keywords: | Heat shock HSP25 HSP27 transfection |
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