| Recombinant Bivalent Vaccine against Foot-and-Mouth Disease VirusSerotype O／A Infection in Guinea Pig |
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| 引用本文： | Yi JZ,Liu MQ,Zhu CZ,Zhang Q,Sheng ZT,Du QY,Yan WY,Zheng ZX. Recombinant Bivalent Vaccine against Foot-and-Mouth Disease VirusSerotype O／A Infection in Guinea Pig[J]. Acta biochimica et biophysica Sinica, 2004, 36(9): 589-596 |
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| 作者姓名： | Yi JZ Liu MQ Zhu CZ Zhang Q Sheng ZT Du QY Yan WY Zheng ZX |
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| 作者单位： | Jian-Zhong YI1,Ming-Qiu LIU1,Cai-Zhu ZHU2,Qiang ZHANG1,Zu-Tian SHENG3,Qing-Yun DU3,Wei-Yao YAN1,and Zhao-Xin ZHENG1* State Key Laboratory of Genetics Engineering,Life Science School,Fudan University,Shanghai 200433,China; 1The Veterinary Institute of Chinese Academy of Agricultural Sciences,Lanzhou 730046,China; 2The Virus Research Institute of Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China |
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| 基金项目： | This study was supported by a grant from the National High TechnologyResearch and Development Program of China (No. 2001AA213071) |
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| 摘 要： | Foot-and-mouth disease (FMD) is a highly contagiousdisease of cloven-hoofed animals such as cattle and pig.The disease causes explosive epidemics and heavyeconomic losses in the agriculture worldwide [1]. FMDvirus (FMDV) shows a high genetic and antigenicvariability, and has seven serotypes: O, A, C, AsiaI, SAT1,SAT2 and SAT3 [2]. The FMDV control is mainly imple-mented using chemically inactivated virus vaccines, whichmay contain residual living virus and pose a risk of virusreleas…
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| 关 键 词： | 重组二价疫苗 口蹄疫 血清型 病毒 天竺鼠 感染 蛋白质 |
Recombinant bivalent vaccine against foot-and-mouth disease virus serotype O/A infection in guinea pig |
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| Yi Jian-Zhong,Liu Ming-Qiu,Zhu Cai-Zhu,Zhang Qiang,Sheng Zu-Tian,Du Qing-Yun,Yan Wei-Yao,Zheng Zhao-Xin. Recombinant bivalent vaccine against foot-and-mouth disease virus serotype O/A infection in guinea pig[J]. Acta biochimica et biophysica Sinica, 2004, 36(9): 589-596 |
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| Authors: | Yi Jian-Zhong Liu Ming-Qiu Zhu Cai-Zhu Zhang Qiang Sheng Zu-Tian Du Qing-Yun Yan Wei-Yao Zheng Zhao-Xin |
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| Affiliation: | State Key Laboratory of Genetics Engineering, Life Science School, Fudan University, Shanghai, China. |
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| Abstract: | In this study, two DNA fragments encoding amino acid (141-160)-(21-140)-(141-160) of the VP1 of FMDV (foot-and-mouth disease virus) serotype O and (138-160)-(21-40)-(138-160) of the serotype A FMDV were chemically synthesized. These two tandem-repeat fragments were ligated and transfected into prokaryotic expression vector pTrcHis A to construct pTH-O-A. The other vector called pTH-O-scIgG-A was constructed similarly only that the two tandem-repeat DNA fragments were linked by the bovine-IgG heavy chain coding sequence. Guinea pigs immunized with the two bivalent vaccines pTH-O-A and pTH-O-scIgG-A showed both specific antibody activity and T cell proliferation responses. FMDV challenge tests showed that 85% and 70% of guinea pigs vaccinated twice with 200 mg of the fusion protein of pTH-O-A were protected from FMDV serotype O and serotype A infection respectively. 70% and 57% of the guinea pigs immunized with the fusion protein of pTH-O-scIgG-A were protected from FMDV serotype O and serotype A infection respectively. |
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| Keywords: | foot-and-mouth disease FMDV serotype O FMDV serotype A VP1 protein bivalent vaccine guinea pig |
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