| G15V点突变对水稻OsRacD基因蛋白产物效应的影响 |
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| 引用本文： | 梁卫红,吴乃虎.G15V点突变对水稻OsRacD基因蛋白产物效应的影响[J].中国生物化学与分子生物学报,2006,22(4):338-342. |
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| 作者姓名： | 梁卫红 吴乃虎 |
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| 作者单位： | 1. 中国科学院遗传与发育生物学研究所,北京,100080;河南师范大学生命科学学院,新乡,453007
2. 中国科学院遗传与发育生物学研究所,北京,100080 |
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| 基金项目： | 科技部科研项目;国家科技攻关项目;河南省高校青年骨干教师资助项目 |
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| 摘 要： | 在水稻OsRacD基因编码GTPase结构域处，采用PCR方法引入G15V点突变模拟GTP结合形式的OsRacD.原核表达并纯化了突变前后的OsRacD蛋白，用于蛋白生化活性的分析.结果显示，突变后的OsRacD蛋白在GTP水解活性上有显著的提高，提示OsRacD在激活前后具有不同的蛋白生化特性，而且可能通过不同的胞内互作蛋白，引发不同的信号传递，证实了OsRacD在Rho信号转导通路中“分子开关”的重要作用.
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| 关 键 词： | OsRacD 点突变 GTPase活性 |
| 收稿时间： | 2005-9-7 |
| 修稿时间： | 2005年9月7日 |
Effects of G15V Point Mutation on Functions of Rice OsRacD Protein |
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| LIANG Wei-Hong,WU Nai-Hu.Effects of G15V Point Mutation on Functions of Rice OsRacD Protein[J].Chinese Journal of Biochemistry and Molecular Biology,2006,22(4):338-342. |
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| Authors: | LIANG Wei-Hong WU Nai-Hu |
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| Institution: | 1)Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China; 2) College of Life Sciences, Henan Normal University, Xinxiang 453007,Henan,China |
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| Abstract: | Point mutation(G15V) was introduced into the GTPase domain of rice OsRacD by PCR to mimic the GTP-binding state OsRacD. OsRacD and G15V-OsRacD proteins were both expressed in E.coli and purified for the biochemical activities analysis. The results showed that OsRacD GTPase activity were enhanced significantly after activated by G15V mutation, suggest that the difference between OsRacD and G15V-OsRacD not only possessed different biochemical activities, but also may interact with different interacting proteins which initiated different signal transduction, and therefore verified OsRacD, as a “molecular switch”, played pivot role in Rho-related pathway. |
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| Keywords: | OsRacD |
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