Generation of high frequency of novel alleles of the high molecular weight glutenin in somatic hybridization between bread wheat and tall wheatgrass |
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Authors: | Heng Liu Shuwei Liu Guangmin Xia |
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Institution: | (1) The Key Laboratory of Plant Cell Engineering and Germplasm Innovation of the Ministry of Education, School of Life Science, Shandong University, 250100 Jinan, People’s Republic of China |
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Abstract: | Somatic hybridization between bread wheat and tall wheatgrass (Agropyron elongatum) has generated fertile introgression progenies with novel combinations of high molecular weight glutenin subunits (HMW-GS).
Most of these novel HMW-GS alleles were stably inherited. Sixteen HMW-GS sequences were PCR amplified from three introgression
progeny lines and sequenced. The alignment of these sequences indicated that five, probably derived from point mutations of
the parental genes, whereas eight likely represent the product of replication slippage. Three Glu-1Ay sequences appear to have lost the transposon presented in the parental gene. Two subunits carry an additional cysteine residue,
which may be favorable to the quality of end-use product. We demonstrate that novel HMW-GS alleles can be rapidly generated
via asymmetric somatic hybridization.
Heng Liu and Shuwei Liu have contributed equally to the work. |
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