Assessment of two immobilized lipases activity and stability to low temperatures in organic solvents under ultrasound-assisted irradiation |
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Authors: | Luciane?Batistella Mara?K?Ustra Aline?Richetti Sibele?B?C?Pergher Helen?Treichel J?V?Oliveira Lindomar?Lerin Email author" target="_blank">Débora?de?OliveiraEmail author |
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Institution: | 1.Departamento de Engenharia de Alimentos,Universidade Regional Integrada do Alto Uruguai e das Miss?es, URI—Campus de Erechim,Erechim,Brazil;2.Departamento de Química,Universidade Federal do Rio Grande do Norte, Centro de Ciências Exatas,Natal,Brazil |
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Abstract: | Both stability and catalytic activity of two commercial immobilized lipases were investigated in the presence of different
organic solvents in ultrasound-assisted system. In a general way, for Novozym 435, the use of ethanol as solvent led to a
loss of activity of 35% after 10 h of contact. The use of iso-octane conducted to a gradual increase in lipase activity in
relation to the contact time, reaching a maximum value of relative activity of 126%. For Lipozyme RM IM, after 5 h of exposure,
the enzyme presented no residual activity when ethanol was used as solvent. The solvents tert-butanol and iso-octane showed
an enhancement of about 20 and 17% in the enzyme activity in 6 h of exposure, respectively. Novozym 435 and Lipozyme IM presented
high stability to storage after treatment under ultrasound-assisted system using n-hexane and tert-butanol as solvents. |
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