|
|||||
|
|
Increasing Accuracy and Throughput in Large-Scale Microsatellite Fingerprinting of Cacao Field Germplasm Collections |
|
| Authors: | Lambert A. Motilal Dapeng Zhang Pathmanathan Umaharan Sue Mischke Michel Boccara Stephen Pinney |
| Affiliation: | 1. Cocoa Research Unit, The University of the West Indies, St. Augustine, Trinidad, Rep. Trinidad and Tobago, West Indies 2. USDA-ARS, BARC, PSI, SPCL, 10300 Baltimore Ave., Bldg. 001, BARC-West, Beltsville, MD, 20705, USA 3. Faculty of Science and Agriculture, Department of Life Sciences, The University of the West Indies, St. Augustine, Trinidad, Rep. Trinidad and Tobago, West Indies 4. CIRAD - UMR DAP TA A96/03, 34398, Montpellier cedex 5, France |
| Abstract: | In this study we report on increasing the rate and accuracy of microsatellite fingerprinting of accessions in Theobroma cacao L. field germplasm collections with a medium-throughput capillary sequencing system. We examined the use of a reduced number of microsatellite loci to decrease the time and materials required for fingerprinting and determined the best available microsatellite loci for accurately separating accessions. A subset of nine informative loci that could separate sixty accessions into the same genetic groupings as a complete set of 37 loci was found. Stringent probability of identity values (i.e. chance of unique accession) was highly influenced (r?=??0.996; P? |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |