Localization of immuno-analogues of erythrocyte protein 4.1 and spectrin in epidermis of psoriasis vulgaris |
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Authors: | Tadamichi Shimizu Yuichi Takakuwa Hiroko Koizumi Teruo Ishibashi Akira Ohkawara |
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Affiliation: | (1) Department of Dermatology, Hokkaido University School of Medicine, Kita 15 Nishi 7, Kita-ku, 060 Sapporo, Japan;(2) Department of Biochemistry, Hokkaido University School of Medicine, Kita 15 Nishi 7, Kita-ku, 060 Sapporo, Japan;(3) Department of Biochemistry, Tokyo Women's Medical College, 8-1, Kawada-cho, Shinjuku, 162 Tokyo, Japan |
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Abstract: | The presence and localization of immuno-analogues of human erythrocyte protein 4.1 and spectrin were examined in the epidermis of psoriasis vulgaris. Immunoblot analysis with antibodies against human erythrocyte protein 4.1 revealed that psoriatic epidermis contains a 4.1-like protein of 80 kDa, and also minor immunoreactive polypeptides, including a 45-kDa polypeptide. The 45-kDa band was not detected in non-lesional epidermis. Lesional epidermis of psoriasis contains spectrin-like proteins of 240 kDa. Analysis with immunofluorescence microscopy revealed that 4.1-like proteins were detected mainly in the cytoplasm of the suprabasal cells in lesional epidermis and in the peripheral cytoplasm of the basal cells in non-lesional epidermis. On the other hand, spectrin-like proteins were localized to the peripheral cytoplasm of basal keratinocytes in both lesional and non-lesional psoriatic epidermis. The present results indicate that proteins related to protein 4.1 and spectrin are consistently detected within epidermal cells of psoriasis, a chronic skin disease characterized by epidermal hyperplasia; the expression and distribution of protein 4.1 in lesional epidermis of psoriasis differs from that in non-lesional epidermis. These membrane skeletal proteins may be of significance in the hyperproliferative epidermis of psoriasis. |
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