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Characterization of a methyl-specific restriction system in Clostridium acetobutylicum strain N1-4081 |
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| Authors: | H Azeddoug J Hubert G Reysset |
| Institution: | Unité des Anaérobies, Institut Pasteur, Paris, France. |
| Abstract: | A type II restriction endonuclease, named CacI, was detected in Clostridium acetobutylicum strain N1-4081. CacI cleaved the tetranucleotide sequence 5' decreases -GATC-3']. The modification system consisted of the methylation of the adenine present in this sequence. CacI, an isoschizomer of MboI, is inactive on dam methylated substrates. |
| Keywords: | Bactopterin Molybdenum cofactor Nicotine dehydrogenase 6-Hydroxynicotinate dehydrogenase 2-furoyl-Coenzyme A dehydrogenase |