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Nucleotide sequence and distribution of the pepPN gene from Lactobacillus helveticus CNRZ32
Authors:Edward G Dudley  Jim L Steele
Institution:Department of Food Science, University of Wisconsin-Madison, 1605 Linden Drive, Madison WI 53706, USA
Abstract:Abstract The Lactobacillus helveticus CNRZ32 gene encoding a di-/tri- peptidase with prolinase activity ( pep PN) was sequenced. An open reading frame of 912 base pairs was identified corresponding to a peptide with a molecular mass of 35.04 kDa. Southern hybridization indicated that the gene sequence is well conserved in strains of lactobacilli and pediococci.
Keywords:Lactobacillus helveticus            Peptidase  Prolinase  Proteolytic enzyme
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