Effects of strigolactone-biosynthesis inhibitor TIS108 on Arabidopsis |
| |
| Authors: | Shinsaku Ito Mikihisa Umehara Atsushi Hanada Shinjiro Yamaguchi Tadao Asami |
| |
| Institution: | 1.Department of Applied Biological Chemistry; The University of Tokyo; Tokyo, Japan;2.Department of Bioscience; Faculty of Applied Bioscience; Tokyo University of Agriculture; Tokyo, Japan;3.Department of Applied Biosciences; Faculty of Life Sciences; Toyo University; Ora-gun, Japan;4.Graduate School of Life Sciences; Tohoku University; Sendai, Japan;5.Japan Science and Technology Agency; CREST; Tokyo, Japan |
| |
| Abstract: | TIS108 is a triazole-type strigolactone (SL)-biosynthesis inhibitor that reduces the level of 2′-epi-5-deoxystrigol (epi-5DS) in rice. Here we report the effects of TIS108 on Arabidopsis. Treatment of TIS108 increased the number of branches and repressed root hair elongation as was observed in SL-deficient mutants, and co-application of GR24, a synthetic SL analog, recovered the TIS108-induced phenotype to that of wild-type. In addition, MAX3 and MAX4 genes in the SL-biosynthesis pathway were upregulated in TIS108-treated Arabidopsis, probably due to feedback regulation caused by SL deficiency. These results indicate that TIS108 is an effective tool for regulating SL production in Arabidopsis. |
| |
| Keywords: | plant hormone strigolactone plant growth regulator biosynthesis inhibitor arabidopsis |
|
|
