Glucose biosensor based on Au nanoparticles-conductive polyaniline nanocomposite |
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Authors: | Xian Yuezhong Hu Yi Liu Fang Xian Yang Wang Haiting Jin Litong |
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Affiliation: | Department of Chemistry, East China Normal University, Shanghai 200062, PR China. yzxian@chem.ecnu.edu.cn |
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Abstract: | In this paper, we report a novel glucose biosensor based on composite of Au nanoparticles (NPs)-conductive polyaniline (PANI) nanofibers. Immobilized with glucose oxidase (GOx) and Nafion on the surface of nanocomposite, a sensitive and selective biosensor for glucose was successfully developed by electrochemical oxidation of H2O2. The glucose biosensor shows a linear calibration curve over the range from 1.0x10(-6) to 8.0x10(-4) mol/L, with a slope and detection limit (S/N=3) of 2.3 mA/M and 5.0x10(-7) M, respectively. In addition, the glucose biosensor system indicates excellent reproducibility (less than 5% R.S.D.) and good operational stability (over 2 weeks). |
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