A minimal medium for growth of Pasteurella multocida |
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| Authors: | Peter E. Jablonski Martha Jaworski Carolyn J. Hovde |
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| Affiliation: | Department of Biological Sciences, Northern Illinois University, DeKalb, IL 60115-2861, USA; Animal and Veterinary Science Department, University of Idaho, Caine Veterinary Teaching and Research Center, Caldwell, ID 83605-8098, USA; Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, ID 83844-3052, USA |
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| Abstract: | Abstract We developed a minimal medium supporting the growth of both toxigenic and nontoxigenic strains of Pasteurella multocida to optical densities of > 0.5 (600 nm ). P. multocida P1059 (ATCC 15742), one of a number of strains which can cause fowl cholera, was used as the model strain in this study. The medium was composed of 17 ingredients including cysteine, glutamic acid, leucine, methionine, inorganic salts, nicotinamide, pantothenate, thiamine, and an energy source. Leucine was not required for growth but was stimulatory, and thiamine could be replaced by adenine. An additional 46 strains of P. multocida were tested, and 40 out of 46 (87%) strains grew as well as strain P1059 through a minimum of 10 serial transfers. P. multocida toxin (PMT) was produced when cells of a known toxigenic strain (P4261) were cultivated in the minimal medium. No growth of Pasteurella haemolytica or Pasteurella trehalosi strains was observed in this minimal medium. |
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| Keywords: | Pasteurella multocida Growth medium |
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