Isolation and Identification of Ice Nucleation Active Fusarium Strains from Rapid Apple Declined Trees in Korea |
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| Authors: | Diane Avalos-Ruiz Leonid N Ten Chang-Kil Kim Seung-Yeol Lee Hee-Young Jung |
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| Institution: | 1.School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea; 2.Department of Horticultural Science, Kyungpook National University, Daegu 41566, Korea; 3.Institute of Plant Medicine, Kyungpook National University, Daegu 41566, Korea |
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| Abstract: | In biological particles such as Fusarium species, ice nucleation activity (INA) has been observed. Fusarium strains isolated from apple declined trees in Korea were identified with a multilocus sequence analysis using the tef1 and rpb1 genes. Droplet-freezing and tube-freezing assays were used to determine the INA of the strains, using Pseudomonas syringae pv. syringae KACC 21200 as a positive control and resulting in seven INA+ fungal strains that were identified as F. tricinctum (KNUF-21-F17, KNUF-21-F18, KNUF-21-F29, KNUF-21-F32, KNUF-21-F38, KNUF-21-F43, and KNUF-21-F44). The effect of Fusarium INA+ KNUF-21-F29 was compared to that of INA– strains on Chrysanthemum morifolium cv. Shinma explants. A higher callus formation and no-shoot formation were observed, suggesting that fungal INA could play a role in cold injuries and be a factor to consider in rapid apple decline. To the best of our knowledge, this is the first report of INA fungal strains isolated in Korea. |
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| Keywords: | cold injury Fusarium ice nucleation activity |
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