Transposition of fly <Emphasis Type="Italic">mariner</Emphasis> elements into bacteria as a genetic tool for mutagenesis |
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Authors: | Mathieu Picardeau |
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Institution: | 1.Unité de Biologie des Spirochètes, Institut Pasteur,Paris Cedex 15,France |
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Abstract: | Mariner eukaryotic elements transpose randomly and independently of any host factors, making them ideal tools for random mutagenesis
in bacteria, including genetically intractable microorganisms. The transposable element Himar1, a member of the mariner family of transposons, originally isolated from the horn fly (Haematobia irritans), has thus been extensively used to generate large numbers of insertion mutants. Transposon-based approaches greatly facilitate
studies of bacterial biology. We summarize the current mariner-based transposon tools and techniques for conducting genetic studies in bacteria. |
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