香石竹植株再生及基因工程研究进展

本文对香石竹的再生体系、遗传转化体系及其分子育种现状作了较为系统的总结。香石竹的再生体系多以器官直接再生不定芽为主，而通过愈伤组织和体细胞胚途径再生报道较少。用农杆菌介导法和基因枪法均可建立香石竹遗传转化体系，但近年来的研究显示农杆菌介导法应用普遍且比较稳定。近年来以延长香石竹瓶插寿命为目标的分子育种研究已取得较大进展，对其色、香和形等其他重要性状的分子育种也已经起步，而有关香石竹抗性的分子育种有待进一步开拓。

Advances in Plant Regeneration and Genetic Engineering of Carnation

This paper reviews plant regeneration, genetic transformation and genetic improvement of carnation (Dianthus caryophyllus). Although there were few reports on plant regeneration from callus and somatic embryogenesis, organogenesis is still the principle method for plantlet regenera- tion of carnation. The genetic transformation systems were established by using Agrobaterium tumefaciens and microprojectile bombardment. The agrobaterium-mediated transformation is a stable and commonly used method for genetic improvement of the species. Great progress has been made in prolonging the vase life of carnation flowers of different cultivars. Some progress has also been made on modification of flower color, flower odor and plant form. Work on improving resistance to pests and diseases should be initiated in the future.