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One-step purification of xylanase from Melanocarpus albomyces and ethylene glycol as a novel soluble additive for enhancing its thermal stability |
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| Authors: | Anshu Gupta Ipsita Roy SK Khare VS Bisaria MN Gupta |
| Institution: | (1) Chemistry Department, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi, 110016, India;(2) Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi, 110016, India |
| Abstract: | Hydrophobic interaction chromatography with Phenyl Sepharose 6 Fast Flow resulted in 7-fold purification of xylanaseactivity from Melanocarpus albomyces with over 60% recovery of theactivity. The purified preparation consisted of two major isoenzymes out of seven present in the organism. Ethyleneglycol, used as the eluent, enhanced the thermal stability of the xylanase activity from a half-life of 2.2 h at 55 °C to almost 40 h. |
| Keywords: | enzyme thermostabilization ethylene glycol Melanocarpus albomyces xylanase purification |
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