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Production of a high molecular weight levan by Bacillus paralicheniformis,an industrially and agriculturally important isolate from the buffalo grass rhizosphere |
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| Authors: | Nasir Anam Ahmad Waqar Sattar Fazal Ashfaq Iram Lindemann Stephen R. Chen Ming-Hsu Van den Ende Wim Ӧner Ebru Toksoy Kirtel Onur Khaliq Shazia Ghauri Muhammad A. Anwar Munir A. |
| Affiliation: | 1.Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering College, Pakistan Institute of Engineering and Applied Sciences (NIBGE-C, PIEAS), Punjab, 38000, Faisalabad, Pakistan ;2.Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, IN, 47907, USA ;3.Laboratory of Molecular Plant Biology, KU Leuven, Leuven, Belgium ;4.IBSB-Industrial Biotechnology and Systems Biology Research Group, Department of Bioengineering, Marmara University, G?ztepe Campus, Istanbul, Turkey ;5.Department of Microbiology, Abbottabad University of Science and Technology, Havelian, Abbottabad, Pakistan ; |
| Abstract: | A new exopolysaccharide (EPS) producing Gram-positive bacterium was isolated from the rhizosphere of Bouteloua dactyloides (buffalo grass) and its EPS product was structurally characterized. The isolate, designated as LB1-1A, was identified as Bacillus paralicheniformis based on 16S rRNA gene sequence and phylogenetic tree analysis. The EPS produced by LB1-1A was identified as a levan, having β(2?→?6) linked backbone with β(2?→?1) linkages at the branch points (4.66%). The isolate LB1-1A yielded large amount (~?42 g/l) of levan having high weight average molecular weight (Mw) of 5.517?×?107 Da. The relatively low degree of branching and high molecular weight of this levan makes B. paralicheniformis LB1-1A a promising candidate for industrial applications. |
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