Homodimeric SV40 NLS peptide formed by disulfide bond as enhancer for gene delivery |
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| Authors: | Bieong-Kil Kim Hyungu Kang Kyung-Oh Doh Seong-Hye Lee Jong-Won Park Su-Jin Lee Tae-Jin Lee |
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| Affiliation: | Department of Physiology, College of Medicine, Yeungnam University, Daegu 705-717, Republic of Korea. |
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| Abstract: | Recently, cysteine residue incorporation increased liposome-mediated transfection compared to unmodified peptide. Therefore, we designed novel modified SV40 NLS peptides, homodimeric (NLS-CTHD, NLS-NTHD) and closed structure (cyclic NLS), simply using disulfide bond between cysteines to develop more efficient and safe non-viral gene delivery system. The simple mix of NLS-CTHD among these novel transfection enhancing peptides with DNA increased the gene transfer potency of cationic liposomes more efficiently with no additional cytotoxicity. |
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| Keywords: | SV40 NLS peptide Gene therapy Liposome Transfection |
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