Metabolic engineering of Escherichia coli for the production of 1-propanol |
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Authors: | Yong Jun Choi Jin Hwan Park Tae Yong Kim Sang Yup Lee |
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Institution: | Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, KAIST, Daejeon, Republic of Korea; BioProcess Engineering Research Center, KAIST, Daejeon, Republic of Korea. |
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Abstract: | An engineered Escherichia coli strain that produces 1-propanol under aerobic condition was developed based on an l-threonine-overproducing E. coli strain. First, a feedback resistant ilvA gene encoding threonine dehydratase was introduced and the competing metabolic pathway genes were deleted. Further engineering was performed by overexpressing the cimA gene encoding citramalate synthase and the ackA gene encoding acetate kinase A/propionate kinase II, introducing a modified adhE gene encoding an aerobically functional AdhE, and by deleting the rpoS gene encoding the stationary phase sigma factor. Fed-batch culture of the final engineered strain harboring pBRthrABC-tac-cimA-tac-ackA and pTacDA-tac-adhE(mut) allowed production of 10.8gL(-1) of 1-propanol with the yield and productivity of 0.107gg(-1) and 0.144gL(-1)h(-1), respectively, from 100gL(-1) of glucose, and 10.3gL(-1) of 1-propanol with the yield and productivity of 0.259gg(-1) and 0.083gL(-1)h(-1), respectively, from 40gL(-1) glycerol. |
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