wiser
tsl
: a recessive X-linked temperature-sensitive lethal mutation that affects the wings and the eyes in Drosophila melanogaster |
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Authors: | Angeliki Mela Sonia G Tsitilou George Yannopoulos |
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Institution: | (1) Division of Genetics, Cell and Developmental Biology, Department of Biology, University of Patras, University Campus, Rio, Patras, 26500, Greece;(2) Present address: Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA;(3) Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Athens, Athens, 15701, Greece |
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Abstract: | In this study, we characterize a recessive X-linked temperature-sensitive mutation of the gene CG32711. The mutation, named wiser
tsl
(wings scalloped-eyes rough), was isolated from a dysgenic cross and is due to a natural P element insertion within the 5′ regulatory region of the gene. Mutant (wiser
tsl
) individuals exhibit wing notching, rough eyes, tarsal malformations and reduced life-span. At 29°C they die at larval and
late pharate stages or during eclosion. The CG32711 (wiser) gene is mainly expressed in the ventral midline cells, the peripheral neural system, the hemocytes and the tracheal system
of embryos. It is also expressed in nurse cells of adult female ovaries. Our results show that the wiser gene is alternatively spliced generating two mRNAs, which share the same open reading frame, while western analysis identified
two protein isoforms. Their expression pattern depends on the stage of development and the culture temperature. wiser
tsl
and wild-type individuals display different expression patterns of the two isoforms and this difference most probably accounts
for the mutant phenotype. Our results indicate that wiser is a vital gene for the development of Drosophila
melanogaster which has no orthologs outside the Drosophilidae.
Sequence data from this article have been deposited with the EMBL/GenBank Data Libraries under accession nos. bankit1003537 EU071463–bankit1003860 EU071464. |
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Keywords: | CG32711 Drosophila melanogaster Temperature-sensitive lethal mutation wiser tsl |
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