Development of a 16S rRNA gene primer and PCR-restriction fragment length polymorphism method for rapid detection of members of the genus Megasphaera and species-level identification |
| |
| Authors: | Ohnishi Akihiro Abe Shinko Nashirozawa Shiho Shimada Sayaka Fujimoto Naoshi Suzuki Masaharu |
| |
| Affiliation: | Akihiro Ohnishi, Department of Fermentation Science, Faculty of Applied Bio-Science, Tokyo University of Agriculture, 1-1 Sakuragaoka 1-chome, Setagaya-ku, Tokyo 156-8502, Japan. a1ohnish@nodai.ac.jp |
| |
| Abstract: | The genus Megasphaera is relevant to the environment, human health and food, and renewable energy for the future. In this study, a primer set was designed for PCR-restriction fragment length polymorphism (RFLP) analyses to detect and identify the members of Megasphaera. Direct detection and identification were achieved for environmental samples and isolates. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
