| 大白菜细胞质雄性不育保持系3411-7--RAPD特异扩增片段的克隆测序 |
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| 引用本文： | 王永飞,马三梅,张鲁刚,. 大白菜细胞质雄性不育保持系3411-7--RAPD特异扩增片段的克隆测序[J]. 广西植物, 2006, 26(3): 300-303 |
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| 作者姓名： | 王永飞 马三梅 张鲁刚 |
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| 作者单位： | 暨南大学,生物工程学系,广东,广州,510632;西北农林科技大学园艺学院,陕西,杨陵,712100 |
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| 基金项目： | 暨南大学校科研和教改项目 |
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| 摘 要： | 利用RAPD技术从大白菜细胞质雄性不育保持系3411-7的DNA中得到一个特异扩增片段MOPB04600。回收该特异扩增片段并将其克隆到pGEM-TEasy载体上进行序列测定。结果表明该片段全长600bp,其碱基组成为A+T=72.33%。通过与GenBank+EMBL+DDBJ+PDB中的455,972个序列进行同源性比较,同源性均小于30%,表明该片段为一新发现的序列。并对该特异片段的来源及其可能的作用进行探讨。
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| 关 键 词： | 大白菜 细胞质雄性不育 RAPD 克隆 测序 |
| 文章编号： | 1000-3142(2006)03-0300-04 |
| 收稿时间： | 2005-01-17 |
| 修稿时间： | 2005-07-22 |
Cloning and sequencing of a specific amplified DNA fragment from cytoplasmic male sterility maintainer line 3411-7 of Chinese cabbage |
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| WANG Yong-fei,MA San-mei,ZHANG Lu-gang. Cloning and sequencing of a specific amplified DNA fragment from cytoplasmic male sterility maintainer line 3411-7 of Chinese cabbage[J]. Guihaia, 2006, 26(3): 300-303 |
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| Authors: | WANG Yong-fei MA San-mei ZHANG Lu-gang |
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| Affiliation: | 1. Department of Bioengineering, Jinan University, Guangzhou 510632, China; 2. College of Horticulture, Northwest Science and Technology University of Agriculture and Forestry, Yangling 712100, China |
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| Abstract: | A specific amplified DNA fragment was obtained from cytoplasmic male sterility maintainer line"3411-7"of Chinese cabbage by RAPD technique. This specific fragment was cloned into pGEM-T Easy vector and DNA sequence was determined. The result showed that it was consisted of 600bp and the base component of A T was 72.33%. Comparison between MOPB04_ 600 and 455,972 sequences from GenBank EMBL DDBJ PPB revealed that the homology were low(less than 30%). This implied that it was a newly found DNA sequence. The origin of MOPB04_ 600 and its possible effects were discussed. |
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| Keywords: | Chinese cabbage(Brassica campestris ssp.pekinensis) CMS RAPD cloning sequencing |
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