Mitochondrial DNA sequences of greenbug (Homoptera: Aphididae) biotypes |
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| Affiliation: | 1. Cesar Australia, 293 Royal Parade, Parkville, Melbourne, Victoria 3052, Australia;2. CABI, Wallingford, UK;3. International Centre of Insect Physiology and Ecology, Nairobi, Kenya;4. Plant Health Australia, ACT 2600, Australia;5. School of Environmental & Rural Science, University of New England, Armidale NSW 2351, Australia;6. School of BioSciences, The University of Melbourne, Victoria 3010, Australia;7. Graham Centre for Agricultural Innovation, Wagga Wagga NSW 2650, Australia;1. School of Biological Sciences, The University of Queensland, St Lucia, Queensland 4072, Australia;2. Koronivia Research Station, Ministry of Agriculture, Nausori, Fiji;3. Vaini Research Station, Ministry of Agriculture Food and Forest, Nuku''alofa, Tonga;4. Crops Division, Ministry of Agriculture and Fisheries, Vaimoso, Apia, Samoa;5. Research Division, Ministry of Agriculture and Livestock, Honiara, Solomon Islands;1. Department of Biology, The University of Western Ontario, London ON N6A 5B7, Canada;2. Biosciences Research Laboratory, USDA-ARS Edward T. Schafer Agricultural Research Center, Fargo, ND 58102, United States;3. Great Lakes Forestry Centre, Natural Resources Canada, Canadian Forest Service, Sault Ste. Marie, Ontario P6A 2E5, Canada |
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| Abstract: | Sequence comparisons were made for 738-bp of mtDNA cloned from seven greenbug, Schizaphis graminum, biotypes (B, C, E, F, G, H and I) obtained from laboratory colonies maintained by USDA-ARS, Stillwater, OK. These sequences include parts of the genes for 16S ribosomal subunit (16S rRNA), tRNAleu, tRNAser, cytochrome b (cytb) and NADH dehydrogenase (ND) subunits one and four. Sequence data revealed considerable variation in 86 (12%) nucleotide sites over the 738-bp sequenced among the seven greenbug biotypes. Nucleotide invariance was observed within the seven greenbug biotypes from both the laboratory colonies and field collected biotype E greenbugs from Kansas, Nebraska, Oklahoma, and Texas. |
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