Trypanosoma cruzi: attachment to perimicrovillar membrane glycoproteins of Rhodnius prolixus |
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Authors: | Alves C R Albuquerque-Cunha J M Mello C B Garcia E S Nogueira N F Bourguingnon S C de Souza W Azambuja P Gonzalez M S |
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Affiliation: | Laboratório de Biologia Molecular e Doen?as Endêmicas, Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, RJ, Brazil. |
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Abstract: | Studies were carried out to identify proteins involved in the interface of Trypanosoma cruzi with the perimicrovillar membranes (PMM) of Rhodnius prolixus. Video microscopy experiments demonstrated high level of adhesion of T. cruzi Dm 28c epimastigotes to the surface of posterior midgut cells of non-treated R. prolixus. The parasites however were unable to attach to gut cells obtained from decapitated or azadirachtin-treated insects. The influence of carbohydrates on the adhesion to insect midgut was confirmed by inhibition of parasite attachment after midgut incubation with N-acetylgalactosamine, N-acetylmannosamine, N-acetylglucosamine, D-galactose, D-mannose or sialic acid. We observed that hydrophobic proteins in the surface of epimastigotes bind to polypeptides with 47.7, 45.5, 44, 43, 40.5, 36, 31 and 13kDa from R. prolixus PMM and that pre-incubation of lectins specifically inhibited binding to 31, 40.5, 44 and 45.5kDa proteins. We suggest that glycoproteins from PMM and hydrophobic proteins from epimastigotes are important for the adhesion of the parasite to the posterior midgut cells of the vector. |
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Keywords: | Chagas disease Rhodnius prolixus (Hemiptera:Reduviidae) Triatomine vector Trypanosoma cruzi (Kinetoplastida: Trypanossomatidae) Peritrophic membranes Hydrophobic proteins Lectins PMM, perimicrovillar membranes GalNAc, N-acetylgalactosamine ManNAc, N-acetylmannosamine GlcNAc, N-acetylglucosamine smallcaps" >d-Gal, smallcaps" >d-galactose smallcaps" >d-Man, smallcaps" >d-mannose NANA, sialic acid WGA, wheat germ agglutinin PNA, peanut agglutinin Con A, concanavalin A PTTH, protoracicotropic hormone HCP, head critical period CBPs, carbohydrate-binding proteins PBS, phosphate-buffered saline FCS, heat-inactivated fetal calf serum 20% BHI, brain heart infusion culture medium TX-114, Triton X-114 Rf, relative mobility |
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