Enzyme-linked immunosorbent assay (ELISA) using a glycolipid antigen for the serodiagnosis of melioidosis |
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| Authors: | Le Van Phung Yuki Han Shiro Oka Hisako Hotta Michael D Smith Prapit Theeparakun Eiko Yabuuchi Ikuya Yano |
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| Institution: | Department of Bacteriology, Osaka City University Medical School, 1-4-54 Asahi-machi, Abeno-ku, Osaka 545, Japan;Department of Microbiology, Hanoi Medical School, 17-Ton That Tung Str., Khuongthuong-Dongda, Hanoi, Viet Nam;Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Bangkok 10400, Thailand;Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Oxford University, Oxford, UK;Department of Medicine, Sappasitprasong Hospital, Ubon Ratchatani, Thailand |
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| Abstract: | Abstract The serodiagnosis of melioidosis is commonly performed with tests using protein or polysaccharide as antigen. However, due to the low sensitivity, specificity and difficulty in the preparation of the antigens, more simple, precise and reproducible diagnostic tests were required. A purified glycolipid antigen (GL) which is a specific lipid component of Burkholderia pseudomallei has been used in an ELISA. With this antigen, specific immunoglobulin G (IgG) was detected in 49 out of 50 melioidosis sera. IgG was also detected in 2 out of 185 (Japanese) and 16 out of 181 (Vietnamese) control sera. Thus, the sensitivity was 98.0%, and specificity was 98.9% and 91.1% in the Japanese and Vietnamese sera, respectively. When the ELISA and indirect haemagglutination (IHA) tests were combined, a sensitivity of 100% and specificity of 97.8% were achieved. The advantages of the glycolipid antigen are ease of preparation, stability, high sensitivity and specificity. |
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| Keywords: | Melioidosis Serodiagnosis Glycolipid antigen ELISA |
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