| 吸入一氧化碳抗肢体缺血/再灌注损伤的实验研究 |
| |
| 引用本文： | 李婧,朱振龙,齐万争,史中立.吸入一氧化碳抗肢体缺血/再灌注损伤的实验研究[J].中国应用生理学杂志,2010(1):33-36,I0001. |
| |
| 作者姓名： | 李婧 朱振龙 齐万争 史中立 |
| |
| 作者单位： | 河北医科大学解剖学教研室;河北医科大学附属第一医院; |
| |
| 基金项目： | 河北省自然科学基金资助项目(C2005000729) |
| |
| 摘 要： | 目的：观察吸入适量一氧化碳（CO）对大鼠肢体缺血/再灌注（I/R）损伤的防治作用。方法：SD大鼠44只,随机分为假手术（S）、I/R、I/R吸入CO（RC）组;通过夹闭股动脉4h、再开放48h,复制肢体I/R损伤模型;RC组行再灌注时,使动物吸入含有CO的医用空气（CO的体积分数为0.05%）,其余两组呼吸正常空气;对比观测缺血肢体大体及骨骼肌组织病理学、缺血肢体湿干重比值（W/D）的变化,流式细胞仪检测肌组织中Bax、Bcl-2的表达水平及细胞凋亡百分比,全自动生化分析仪检测血清乳酸脱氢酶（LDH）和肌酸激酶（CK）的变化。结果：与I/R组比较,RC组动物W/D、血清LDH及CK含量、肌组织中Bax表达水平及细胞凋亡百分比均显著降低,肌组织Bcl-2表达水平显著升高,缺血肢体大体观及肌组织病理学明显改善。结论：吸入适量浓度的外源性CO对肢体I/R损伤有防治作用。
|
| 关 键 词： | 一氧化碳 肢体 缺血/再灌注损伤 大鼠 |
Carbon monoxide inhalation protects against limb ischemia/reperfusion injury |
| |
| LI Jing,ZHU Zhen-long,QI Wan-zheng,SHI Zhong-li.Carbon monoxide inhalation protects against limb ischemia/reperfusion injury[J].Chinese Journal of Applied Physiology,2010(1):33-36,I0001. |
| |
| Authors: | LI Jing ZHU Zhen-long QI Wan-zheng SHI Zhong-li |
| |
| Institution: | LI Jing1,ZHU Zhen-long2,QI Wan-zheng1,SHI Zhong-li1(1.Department of Anatomy,2.the First Affiliated Hospital,Hebei Medical University,Shijiazhuang 050017,China) |
| |
| Abstract: | Objective:To study the protective effect of carbon monoxide(CO) inhalation in the limb ischemia/reperfusion(I/R) injury of rats.Methods:Forty-four Sprague-Dawley rats were randomly divided into three groups:S,I/R and RC groups.I/R injury model was made by the occlusion of the femoral artery for four hours and the reperfusion for forty-eight hours.RC group was exposed to medical air mixed CO (the volume fraction of CO:0.05%) during limb reperfusion in rats.The other two groups were exposed to the routine air... |
| |
| Keywords: | carbon monoxide limb ischemia/reperfusion injury rat |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
