Regeneration of Transgenic Plants from Electroporated Protoplasts of Asparagus officinalis L |
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| Authors: | Sandip Mukhopadhyay Serge Overney Serge Yelle Yves Desjardins |
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| Affiliation: | 1. Centre de Recherche en Horticulture, Departement de Phytologie, F.S.A.A., Pavilion Envirotron, University Laval, Québec G1K 7P4, Canada
3. Laboratoire de Biochimie et de Physiologie Végétales, Univers1te de Genève, 3place de I’Université, 1211, Geneve 4, Suisse
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| Abstract: | An effective method for consistent regeneration of transgenic asparagus (Asparagus officinalis L) plants from electroporated protoplasts is described. Transgenic plants containing β-glucuronidase (GUS) and neomycin-phosphotransferase (NPT II) genes were obtained by electroporating callus-derived protoplasts of Asparagus officinalis L. Embryogenic callus tissue and plants from four kanamycin resistant lines expressed P-glucuronidase activity, as revealed by histological staining. The amplification of genomic DNA by polymerase chain reaction revealed the presence of both GUS and NPT II genes in transformed callus tissue and plants. Southern hybridization confirmed the integration of these genes into the asparagus genome. |
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