Effects of Septal Lesions on Enzymes of Acetyl-CoA Metabolism in the Cholinergic System of the Rat Hippocampus |
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| Authors: | Andrzej Szutowicz Paul A Srere Charles N Allen Isaac L Crawford |
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| Institution: | Preclinical Science Unit, Department of Biochemistry, The University of Texas Health Science Center, Dallas, Texas, U.S.A.;Laboratory of Neurophysiology, Veterans Administration Medical Center, Department of Neurology, The University of Texas Health Science Center, Dallas, Texas, U.S.A.;Departments of Pharmacology, The University of Texas Health Science Center, Dallas, Texas, U.S.A. |
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| Abstract: | Abstract: Electrolytic lesions made in the medial septum of the rat brain caused an 80% decrease in the activity of choline acetyltransferase and a 33% reduction in ATP-citrate lyase activity in the synaptosomal fraction from the hippocampus. Decreases in the activities of the two enzymes in the cytosol (S3) fraction were 70 and 13%, respectively. The activities of pyruvate dehydrogenase, citrate synthase, acetyl-CoA synthase, and carnitine acetyltransferase in crude hippocampal homogenates and in subcellular fractions were not affected by septal lesions. The data indicate that ATP-citrate lyase is linked to the septal-hippocampal pathway and that the enzyme is preferentially located in cholinergic nerve endings that terminate within the hippocampus. |
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| Keywords: | ATP-citrate lyase Choline acetyltransferase Septal-hippocampal pathway Acetyl-CoA |
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