水稻高叶绿素含量基因DET1 cDNA的克隆、生物信息学分析及超表达载体的构建

提高水稻叶片中叶绿素含量可以提高光合作用效率。以水稻高叶绿素含量突变体为材料，在前期精细定位的基础上，克隆了高叶绿素含量基因DET1（DE-ETIOLATED 1）cDNA。测序结果显示：DET1基因全长cDNA为1536 bp，由11个外显子组成，编码512个氨基酸。与野生型珍汕97B相比，DET1基因在第7个外显子上有T-C的转换，该转换引入了一个EcoR I酶切位点，并导致第328位的亮氨酸（L）改变为丝氨酸（S），且突变位点处于高度保守区域内。DET1基因的氨基酸序列与玉米等其它高等植物的氨基酸序列同源性高达62%以上。利用生物信息学方法对DET1基因的序列、进化树、理化性质及亲疏水性等进行分析，并构建了水稻超表达载体pCUPHN-DET1，为水稻高叶绿素含量突变基因DET1的功能验证奠定基础。

cDNA Cloning,Bioinformatics Analysis and Construction of Overexpression Vector of High-chlorophyll Rice Gene DET1

The efficiency of photosynthesis can be improved by increasing chlorophyll content in rice leaves.DET1(DE-ETIOLATED 1)gene related to high chlorophyll content has been cloned based on the fine mapping and sequence analysis of the DET1 gene was carried out in the high-chlorophyll mutants.The DET1 cDNA full-length of 1536 bp encoded 512 amino acids,and revealed a single T-to-C base transversion in the seventh exon of the DET1 gene which consisted of 11 exons,in comparison with the normal wild-type sequence.Th...