Characterization of human thyrotropin receptor-related peptide-like immunoreactivity in peripheral blood of Graves' disease. |
| |
| Authors: | M Murakami K Miyashita M Yamada T Iriuchijima M Mori |
| |
| Institution: | First Department of Internal Medicine, Gunma University, School of Medicine, Maebashi, Japan. |
| |
| Abstract: | An antiserum raised against an alignment of amino acid-(32-56), termed TSHRP-1, in the extracellular domain of human thyrotropin (TSH) receptor was used to identify the TSH receptor-like substance in plasma of Graves' disease. The dilution curve of plasma TSHRP-1-like immunoreactivity was observed in a manner parallel to the standard synthetic peptide curve in radioimmunoassay, and its molecular weight estimated approximately 60 kDa. The amounts of TSHRP-1-like immunoreactivity were significantly higher in Graves' plasma than those in plasma of normal and hypothyroid patients due to Hashimoto's thyroiditis. The present results indicate that human peripheral blood possesses a soluble form of the extracellular domain of TSH receptor which may contribute to the pathophysiology of Graves' disease. |
| |
| Keywords: | |
|
|
