Isozyme and morphological characteristics of the anaerobic fungus Piromyces mae isolated from the duodenum, rumen and faeces of sheep |
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| Authors: | M.V.L. Wong Y.W. Ho S.G. Tan N. Abdullah S. Jalaludin |
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| Affiliation: | Department of Animal Science, Faculty of Science and Environmental Studies, Unirersiti Pertanian Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Department of Biology, Faculty of Science and Environmental Studies, Unirersiti Pertanian Malaysia, 43400 UPM Serdang, Selangor, Malaysia; Department of Biochemistry and Microbiology, Faculty of Science and Environmental Studies, Unirersiti Pertanian Malaysia, 43400 UPM Serdang, Selangor, Malaysia |
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| Abstract: | Abstract Isolates of anaerobic fungi obtained from the rumen, duodenum and faeces of sheep were identified as Piromyces mae based on their morphological characteristics observed using light microscopy. There was no significant morphological variation among the isolates of P. mae from the rumen, duodenum and faeces. Isozymes of 12 isolates of P. mae (one each from the rumen, duodenum and faeces from 4 different sheep) were analysed by PAGE. A total of 12 isozymes were studied and 5 isozyme loci were successfully typed. They were malic enzyme, malate dehydrogenase, shikimate dehydrogenase, α-esterase and β-esterase. All the isolates of P. mae regardless of whether they were from the rumen, duodenum or faeces or from different animals produced very similar isozyme banding patterns for each of the enzyme systems. The similar isozyme profiles of the isolates indicate that they are of the same species although they exist in different regions of the alimentary tract. |
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| Keywords: | Anaerobic fungus Isozyme Rumen Duodenum Faeces Piromyces mae |
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